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Cys-NEM Samples were treated to a final concentration of 20 mM N-ethyl maleimide (NEM) to alkylate Cys

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Clinical trials showed a favorable safety and tolerability profile with no serious adverse events

10.1016/s0140-6736(11)60178-5 101 LiC.MiaoX.WangS.LiuY.SunJ.LiuQ.et al (2021a)

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3,15,17,2030 Dose and pattern of use Zimmermans therapeutic misadventure describes acute liver injury following APAP consumption and failure of APAP therapy, when used at or below the daily maximum recommended dose of 4000 mg