Spermatozoa have the highest selenium content in mammalian tissues, and the need for selenium increases at the onset of spermatogenesis ( 2.5 GPX5 Glutathione peroxidase 5 (GPX5), an epididymis-specifically expressed selenium-free antioxidant enzyme, was first isolated and identified in the epididymal tissues of mice ( During epididymis transport, spermatozoa are vulnerable to intracellular and extracellular ROS attacks and rely on antioxidant scavengers synthesized by the epididymis for protection
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Arthur JR (2000) The glutathione peroxidases
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Cell number changes in Alzheimer's disease relate to dementia, not to plaques and tangles
Once eluted, proteins were further concentrated with filters and then purified by anion exchange chromatography on HitrapQ (GE Healthcare) and collected fractions were further loaded onto size exclusion column (Superdex 75 16/60, GE Healthcare)
