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Bacteriostatic water is required for multi-dose vials because the 0.9% benzyl alcohol inhibits bacterial growth after the vial is punctured and exposed to airborne contaminants during syringe draws

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TMAO activated the expression of the inflammatory mediator high mobility group box 1 (HMGB1), which stimulated multiple receptors such as toll-like receptor 4 (TLR4), thus mediating extracellular signal-regulated kinases 1 and 2 (ERK1 and ERK2) and the NF-B signaling pathway (82)

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