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It is confirmed in mouse myocardial microvascular endothelial cell line H5V that UCB may influence the regulatory pathway of NF-B via interaction with IKK proteins by inhibiting tnf-stimulated NF-B nuclear translocation (90)

The conditions, such as a 75 mM sodium phosphate buffer (pH 3.0) with 1.7 mM 1-octanesulfonic acid, 25 M EDTA, 10% methanol, and a flow rate of 1 mL/min, are optimized for precision and sensitivity, ensuring accurate neurotransmitter profiling at a column temperature of 25C

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Secondary antibodies (Goat anti-rabbit IgG Alexa Flour 647, Goat anti-rabbit IgG Alexa Flour 488, Goat anti-mouse IgG Alexa Flour 488 and Goat anti-rat IgG Alexa Flour 647) were applied for 1 h room temperature

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