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Cells were washed three times with 1 ml PBS and lysed with 200 l 0.5% (v/v) Triton X-100 (Fisher Scientific) at 37 C for 5 min, followed by the addition of 800 l PBS

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The peptide's activation of ERK1/2 and downstream transcription factors including Egr-1 drives expression of collagen synthesis genes while its enhancement of cellular migration ensures efficient fibroblast recruitment to sites requiring matrix deposition, creating optimal conditions for rapid yet organized tissue reconstruction

If it doesnt clear up after 30 minutes of gentle rolling, it should be discarded

More importantly, if you are using a 1 mL (100 unit) syringe, there is very little room for higher doses

Consistency across vial sizes eliminates confusion when you switch between them or compare notes with others using different vial sizes