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These cells are nourished by a neovasculature and are persistent at the MI site, where they are metabolically active expressing components requisite to angiotensin (Ang) peptide generation, including converting enzyme, receptors for AngII and transforming growth factor (TGF)-beta1

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The goal is to let the hypothalamic-pituitary axis adjust gradually

Ice crystal formation physically damages the peptide structure, creating aggregation and reducing biological activity