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After 2 days of expression, cells were harvested in ice-cold PBS and subsequently lysed in AP-MS buffer (10 mM Tris/Cl pH 7.5, 150 mM NaCl, 10% Glycerol, 0.5 mM EDTA, 0.5% Nonidet P40) supplemented with protease inhibitor cocktail (Roche, Rotkreuz, Switzerland) by sonification, followed by centrifugation (20,000 g for 10 min

Usually a horizontal inframammary incision is not necessary

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The detection of Fusobacterium and other anaerobic taxa in peripheral blood around parturition suggested that intestinal or oral barrier compromise might allow for the systemic dissemination of these microbes, which subsequently colonize the uterus 129