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Seventeen differential metabolites were identified, including cellobiose, chenodeoxycholic acid 3-sulfate, D-alanine, D-alanyl-d-alanine, glycoursodeoxycholic acid 3-sulfate, diaminopimelic acid, D-glutamic acid, deoxyglycocholate, phenylpyruvic acid, urobilinogen, L-homoserine, N2-succinyl-ornithine, hydroxypropionic acid, indoleacetic acid, taurodeoxycholic acid, N-methylnicotinamide, and glycocholic acid (Table S2)

Meanwhile, mRNA levels of individual TET family members ( TET1 TET3 ) remained largely comparable between shCtrl and shGDH1 cells (Extended Data Fig