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The change in pH of TSB and 1 PBS at different GSH concentrations was determined using a pH meter (Mettler-Toledo GmbH, Greifensee, Switzerland) calibrated with pH standards of 4.0 and 7.0 and the pH values of TSB and 1 PBS as a function of GSH concentration reported in Table 2

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To assess the G/D-CuPs biocompatibility and its ability to support cell growth, we performed in vitro three-dimensional (3D) culture experiments using NIH/3T3 cells as a model