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Crystallization during freezing and thawing can damage the peptide structure

The mission of the MHPU is to enhance community policing efforts, have positive interactions with community members and visitors to the Capitol Complex, suppress civil disturbances, and deter terrorism and criminal activity

[DOI] [PMC free article] [PubMed] [Google Scholar] De Oliveira D

After adjusting the protein concentration using RIPA buffer, an equal amount of total protein (20 g) was resolved on 5% acrylamide gels (Amresco), and proteins were electrophoretically separated on SDS gels (1015%, Sigma) at a constant voltage of 120 V for 20 min ( n = 6 per group)

Patrushev, N., Seidel-Rogol, B

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