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Limitations This studys strengths comprise the tightly controlled protocol and crossover randomized controlled trial design, which allowed for direct within-subject repeated-measures investigation of the effects of total sleep deprivation and ammonia inhalation

World J Gastroenterol 2006

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The dialysate samples were treated with 40 mM perchloric acid (PCA) and 5 mM EDTA before subjected to the high-performance liquid chromatography (HPLC)