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We reasoned that evolution selected individuals triggering antimicrobial resilience together with the pool of biofilm-related genes, including ROS detoxification and changes to prevent ROS production

S.et al (2014a)

However, there are several limitations to these models, including: (1) lengthy observation periods, (2) high cost, (3) difficult genetic manipulations, and (4) ethical concerns regarding prolonged and repeated injury of a large number of mammals

Salting-out extraction of allicin from garlic ( Allium sativum L.) based on ethanol/ammonium sulfate in laboratory and pilot scale