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Briefly, HaCaT keratinocytes were seeded at a density of 10,000 cells per well in 200 l fresh culture medium (DMEM supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin solution) into 96-well flat-bottomed microtitre plates (Costar, Corning, USA) and incubated at 37 C in humidified 5% CO 2 using AutoFlow NU-5510 Direct Heat CO 2 Incubator (NuAire, USA) for 24 hours before treatment

Nakano, Y

Cryotherapy and Localized Cryotherapy Cryotherapy involves exposing the body to extremely cold temperatures for a short duration, which can stimulate a range of physiological responses

NPN: 80098063

10.1016/S0969-2126(99)80023-9 Structure

Cancer 22 FloreanC.SchnekenburgerM.GrandjenetteC.DicatoM.DiederichM