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Consequently, adequate numbers of explants were placed on coverslips, which were placed in 6-well culture plates or into cell culture dishes in cell culture medium 199 containing fetal calf serum (FCS), bovine pancreatic insulin, human transferrin, hydrocortisone, and antibiotic/antimycotic solution for P/S cell analysis or cell counting studies, respectively (22)

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BMC Med Res Methodol