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One individual per group was euthanized before addressing the next animal in each group to render differences arising from the time of day at euthanization (e.g., cecal mass changes resulting from differences in time post-food consumption) comparable across all groups

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The efficient delivery of 5moU-modified Nanoluc mRNA has also been demonstrated in pigs: the mRNA was detected in the liver, stomach, small and large intestines, spleen, lungs, uterus, red bone marrow, and blood cells, with no signs of hepatotoxicity [288]

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