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thermophilus S-3 was cultured in M17 medium supplemented with 2% lactose (LM17) at 37C

IGF-1 LR3 binds the alpha subunit of the IGF-1 receptor tyrosine kinase, inducing autophosphorylation of the beta subunit on specific tyrosine residues (Y1131, Y1135, Y1136) that create docking sites for insulin receptor substrate (IRS) proteins and Shc adaptor proteins

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Moore S, Hill EM, Dyson L, Tildesley MJ, Keeling MJ