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Islets were freshly isolated via collagenase digestion of pancreata as described previously 43

10.1016/j.jbiotec.2006.03.002

[DOI] [PMC free article] [PubMed] [Google Scholar] 15.Vincenzi B., Daniele S., Frezza A.M., Berti P., Vespasiani U., Picardi A., Tonini G., Vincenzi B., Daniele S., Frezza A.M., et al

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When Wnt pathway activation occurs, Twa1 translocates to the nucleus, and its conserved CRA (CT11-RanBPM) domain is required for its interaction with -catenin, which promotes -catenin nuclear retention and increases Wnt/-catenin downstream target gene expression