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BioMed Research International

2019 Jun 19;10:676

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Immunoprecipitation Hepatocytes were lysed in an immunoprecipitation (IP) buffer (150 mM NaCl), 50 mM Tris-HCl, 1% Nonidet P-40, pH 7.8, and a mammalian cell-specific protease inhibitor cocktail

Recent methods and variations in methodology Lung lymphocytes have been purified on both the normal density barrier [32] and a slightly denser barrier of approx 16.5% (w/v) iodixanol (described in the paper as 27.5% (v/v) OptiPrep) [33] similar to that used for intestinal cells [34]