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Suitable fractions were pooled, concentrated with Amicon Ultra 10 kDa filters (Merck Life Science Ltd, Gillingham, UK) and diluted in 20 mM Tris-HCl, pH 7.8, 0.5 M NaCl and 5 M urea buffer for storage at -80 C

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American College of Cardiology/American Heart Association Task Force on Practice, S