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The cleavage of the pro-domain is not required for protease activity
HeLa cells were treated by nocodazole at 37℃ for 20 hours or treated by ATP at 30℃ for 1 hour
The cells were 4% paraformaldehyde fixed (15 min) and then incubated in 10% normal goat serum for 1h to permeabilise the cells and block non-specific protein-protein interactions
MB10415-100
subcellular location:Present at the centrosomes at interphase
CK-5 Polyclonal Antibody - E-AB-10147 Intracellular delivery of proteins The cleavage of the pro-domainCK 5 Polyclonal Antibody Sizes: 60L, 120L, 200L Catalogue Numbers: E AB 10147 60, E AB 10147 120, E AB 10147 200 Citations, Manuals and MSDS Available upon request. Abbreviation: KRT5 Target Synonym: 58 kDa cytokeratin; CK 5; CK5; Cytokeratin 5; Cytokeratin5; DDD; DDD1; EBS2; epidermolysis bullosa simplex 2 Dowling Meara Kobner Weber Cockayne types; K2C5; K5; keratin 5 (epidermolysis bullosa simplex; Dowling Meara Kobner Weber Cockayne types); Keratin