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The representative nature of these samples with respect to the entire lung was established by comparing the densities of the sampled sites with the frequency distribution of the densities in the entire lung on multidetector CT as reported in McDonough et al .[12]

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Non-invasive techniques, including high-intensity focused ultrasound, also face the challenge of recurrence [11]

Total RNA extraction was performed using the AG RNAex Pro reagent (Accurate Biology, Changsha, China) following the manufacturer's protocol

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This is not well understood mechanistically but may relate to individual sensitivity, dehydration, or unrelated factors coinciding with supplement use