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In discussions of injectable timing, some prefer evening administration, but there is no validated, approved protocol, and the consensus emphasis is that consistency matters more than exact timing

Further study found that the expression of exogenous prominin 2 inhibited cell ferroptosis induced by RSL3, ML210, FIN56, and erastin

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Western Blotting Analysis Proteins from the whole kidney lysates were separated on a 10% SDS-polyacrylamide gel, transferred onto PVDF membrane, blocked in 5% BSA for 60min, and then primary antibodies against KIM-1, IL-1, TNF-, Ferritin, FPN, xCT, and p53 at the dilution of 1:1,000, as well as 4-HNE, GPX4, and -actin at the dilution of 1:4,000 were probed at 4C overnight