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The cells were harvested and disrupted at 4C with 425600-m (diameter) glass beads in buffer G [10 mM Tris-HCl (pH 7.5), 300 mM sorbitol, 100 mM NaCl, 5 mM MgCl 2 , 1 mM EDTA, and 1 M pepstatin A]

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