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Chromatographic separation was conducted on a Brownlee SPP C18 column (2.1 mm 75 mm, 2.7 m particle size, Perkin Elmer, Waltham, MA) using mobile phase A (100% H2O containing 0.1% formic acid and 1% of 1 M NH 4 OAc) and B (1:1 acetonitrile:isopropanol containing 0.1% formic acid and 1% of 1 M NH 4 OAc)

N -acetylcysteine: antioxidant, aldehyde scavenger, and more

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Still, in the process, it can create Superoxide, oxygen with too many electrons that is poisonous to everything around it, be it mitochondria, DNA, proteins, cell membranes, etc

It exerts potent antioxidant effects, modulates cellular signaling, and supports cognitive function, cell viability, and adaptation to stress