This creates a net negative balance within adipocytes, resulting in decreased lipid droplet size and eventual adipocyte shrinkage
10.1016/j.ejphar.2013.01.026 94 NusserZ.LujanR.LaubeG.RobertsJ
Define a measurable outcome: weight, waist circumference, recovery time, or cognitive test
RNA extraction and RTqPCR Total RNA from cells was isolated via TRIzol (Takara, Otsu, Japan) and subsequently purified with chloroform/isopropanol/ethanol, and cDNA was synthesized via the PrimeScript RT reagent Kit (Takara) according to the manufacturers instructions and used as the template for qRTPCR
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The NorthStar Ambulatory Assessment in Duchenne muscular dystrophy: considerations for the design of clinical trials