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Working with cold solutions can interfere with complete dissolution and may affect the peptide's interaction with the diluent

doi: 10.1111/j.1365-2982.2012.01891.x 201 KomaroffALLipkinWI

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PBS should therefore be selected based on experimental requirements rather than convenience

Touching or rubbing the injection site Touching or rubbing the injection site can lead to irritation, potential infection, or damage