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Following this, the solution was subjected to dialysis using a dialysis bag with a molecular weight cutoff of 8000-14000 Da

Protection from light is also specified because UV radiation can photo-reduce Cu(II) to Cu(I), which has different coordination chemistry and generates reactive oxygen species that degrade the peptide backbone

When theyre done with the bandaging, slowly rotate your arm over your head in a circular motion 9-10 times to get the blood flowing