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Consequently, the yield was calculated using this purity, 66%, from the concentrations of -EC determined by HPLC analysis using the standard -EC with assumed purity, 100%

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Beads were then washed three times with binding buffer, and bound complexes were eluted in 20 mM Tris-HCl pH 8.0, 150 mM NaCl, 250 mM imidazole elution buffer

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