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Canonical markers of necroptosis a

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The outer solution of the dialysis bag was stirred at 60 rpm and maintained at a temperature of 37 C

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Although a gold standard in exogenous substances behavior studies, Caco-2 cell monolayers exhibit certain limitations such as a much higher transepithelial electrical resistance (TEER, up to 500 cm 2 ) compared to the human intestine (1269 cm 2 ), overestimated P-gp-mediated efflux and low paracellular permeability, a lack of metabolic mucus and enzymes at their apical side, which limits the relevance of the Caco-2 cell line in metabolism studies under standard culture conditions (Thummel, 2007)