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HEK-293T cells were grown in high glucose (4.5 g/L glucose) Dulbecco's modified Eagle's medium (DMEM, Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS, Natocor), penicillin/streptomycin (100 units/mL and 100 g/mL, respectively, Thermo Fisher Scientific) and 110 mg/L of sodium pyruvate (Thermo Fisher Scientific) in a 37 C humidified incubator containing 5% CO 2

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Never touch the stopper with bare fingers

The ferroptosis-NLRP1 inflammasome: the vicious cycle of an adverse pregnancy

10.1038/362709a0 Nature 111 LiuJ.WangR.HuangB.LinC.ZhouJ.PanX