Pathogenic role of Th17 cells in autoimmune thyroid disease and their underlying mechanisms
Lunkes LC, Paiva IM, Egger RC, Braga WF, Alvarez-Leite JI, da Cunha Barreto-Vianna AR, Murgas LDS (2021) Melatonin administration attenuates acute stress by inducing sleep state in zebrafish (Danio rerio)
Follow institutional protocols for reconstitution and aseptic handling
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49 NakamichiI.TomitaY.ZhangB.SugiyamaH.KanakuraY.FukuharaS.et al (2007)
Eluates were pooled together and concentrated down to around 75 L using an Amicon Ultra 0.5 mL 3000 NMWL centrifugal filter (Millipore UFC500324), supplemented with 25 L of NuPage 4x LDS sample buffer (Invitrogen, NP0007) containing 100 mM DTT