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For luciferase reporter vectors, PRKAA1 5UTR or scramble 5UTR DNA sequences were synthesized and cloned into pCDNA3 RLuc POLIRES FLuc (Addgene, 45642)
Qin Z, Fisher GJ, Voorhees JJ, Quan T
Thus, the AC/FC ratio is a measure of acylated carnitines versus the free carnitines
Those like, yes, they had less than their peak after they'd been doing it for a year, but it had this protective effect against age related decline and sarcopenia, because the other two groups, the moderate group kind of probably didn't, wasn't statistically powered enough to pick out some of the differences
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